1.
Sensitivity of PCR assays and characteristics of standard curves for 16 type-specific PCRs constructed with plasmids containing the entire genome of the different HPV types
| Gene | Sensitivity (HPV copies) | Standard curves | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MY09/11 PCR | Type-specific PCRs | |||||||||||||
| Slope | Efficiency | Value of fit (R2) | ||||||||||||
| HPV 16 E7 | 102 | 10 | 3.4836 | 1.940 | 0.9991 | |||||||||
| HPV 18 E7 | 102 | 10 | 3.3632 | 1.928 | 0.9982 | |||||||||
| HPV 31 E6 | 10 | 1 | 3.4508 | 1.977 | 0.9998 | |||||||||
| HPV 33 E6 | 102 | 10 | 3.4632 | 1.912 | 0.9954 | |||||||||
| HPV 35 E6 | 104 | 10 | 3.3551 | 1.974 | 0.9995 | |||||||||
| HPV 39 E7 | 102 | 10 | 3.5730 | 1.932 | 0.9965 | |||||||||
| HPV 45 E7 | 102 | 1 | 3.1596 | 1.835 | 0.9755 | |||||||||
| HPV 51 E7 | 105 | 1 | 3.3482 | 2.000 | 0.9979 | |||||||||
| HPV 52 E7 | 104 | 10 | 3.3143 | 1.995 | 0.9990 | |||||||||
| HPV 53 E6 | 104 | 10 | 3.4650 | 1.918 | 0.9999 | |||||||||
| HPV 56 E7 | 104 | 10 | 3.1775 | 1.961 | 0.9953 | |||||||||
| HPV 58 E6 | 102 | 10 | 3.5450 | 1.919 | 0.9962 | |||||||||
| HPV 59 E7 | 102 | 10 | 3.6836 | 1.860 | 0.9995 | |||||||||
| HPV 66 E6 | 102 | 10 | 3.5661 | 1.927 | 0.9977 | |||||||||
| HPV 67 L1 | 103 | 102 | 3.4973 | 1.835 | 0.9968 | |||||||||
| HPV 68 E7 | 103 | 10 | 3.2000 | 2.044 | 0.9998 | |||||||||