Fig 1.

Apoptotic cells induce increased TSP-1 expression in ECs. (A) HUVEC or HMEC-1 cells were exposed to apoptotic eEND2 cells for 4 hrs and differences in the expression of TSP-1 transcript was measured by real-time qPCR (n= 8). (B) HUVEC were exposed to apoptotic eEND2 cells for three and for 8 hrs and TSP-1 protein in the supernatant was measured by ELISA (n= 4). (C) Pre-incubation with cytochalasin D did not prevent apoptotic-cell-induced increase in TSP-1 mRNA expression in HUVEC (n= 3). Data are given as mean ± S.D. (*P < 0.05 **P < 0.001).