Skip to main content
. 2010 Aug 19;14(7):1922–1934. doi: 10.1111/j.1582-4934.2009.00799.x

Fig 4.

Fig 4

Supernatant of HUVEC exposed to apoptotic eEND2 cells activates monocyte-derived macrophages and U937 cells. (A) Human monocyte-derived macrophages were exposed to supernatant of HUVEC exposed to apoptotic eEND2 cells, to supernatant of untreated HUVEC or to rh M-CSF for 3 hrs and expression level of different cytokines and chemokines were determined by real-time qPCR. Plotted are the average expression levels of three experiments with macrophages derived from different donors. Note the different y-scale for expression of IL-8. (B) U937 mRNA expression profiles of the same set of cytokines/chemokines after stimulation for 3 hrs with supernatant of untreated HUVEC, HUVEC exposed to apoptotic cells and PMA (n= 3). Note the different y-scale for expression level of MCP-1, TNF-α and IL-10. GoI: Gene of interest.