Fig 6.

Migration of macrophages or U937 cells is influenced by TSP-1. HUVEC were seeded into the lower compartment of transwell devices and exposed to apoptotic eEND2 cells for 8 hrs or left untreated. Fluorescence-labelled macrophages or U937 cells were added to the upper compartment. In some experiments macrophages or U937 cells were pre-incubated with RGD peptides (50 μM) or TSP-1 in the supernatant was blocked by neutralizing antibodies (5 μg/ml). After 60 min. transmigration of macrophages or U937 cells through the transwell membrane was counted using fluorescence microscopy. Results are given as mean ± S.D. of at least three independent experiments (*P < 0.05).