Fig 4.

ELISA results. (A) OD signal from ELISA against K562 – OD signal from ELISA against Lymphocytes. (B) (K562-background)/(Lymp-background). Phage diluted from stock preparation, approximately 10¹³ phage titre. Background is ELISA signal against blank control (no cells coated). Zero is no phage added in the ELISA. Wells were coated with approximately 10,000 cells per well and treated with methanol and paraformaldehyde prior to blocking. ELISA made in duplicates. S1.1D8, S2.4D1 and S3.2G2 were chosen based on stronger or similar binding to lymphocytes compared to K562 in initial screening. S2.1A8, S3.1A9 and S3.1B2 were found to bind stronger to K562 compared to lymphocytes in a concentration dependent manner.