Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jan 27;16(2):260–272. doi: 10.1111/j.1582-4934.2011.01289.x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 The Authors Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd

PMC Copyright notice

Fig 3 — Modulation of Cldn1 and Cldn4 expression in vitro. (A) Expression of claudins (1, 4) in CC531 cells co-cultured for 24 to 72 hrs with Kupffer cells (KCs) and hepatocytes (HCs) in comparison to the housekeeping gene γ-tubulin as shown by RT-PCR. (B) Expression of claudins (1, 4) in CC531 cells harvested from round and flat flasks as shown by RT-PCR. Lanes 1, 3, 6: CC531 cells harvested from round bottles after 24, 48 and 72 hrs, respectively. Lanes 2, 5, 8, 10: CC531 cells harvested from flat flasks after 24, 48, 72 and 96 hrs, respectively. Lanes 4, 7, 9: CC531 cells harvested from flat flasks after being transferred from round bottles after 24, 48 and 72 hrs, respectively. (C) Down-regulation of claudins (1, 4) in CC531 cells after siRNA transfection as shown by RT-PCR. (D) Down-regulation of Cldn4 after 24–72 hrs in CC531^si.Cldn1 cells (compared to CC531^nonsense cells) as shown by RT-PCR.