Table 2.
Table of antibodies
| Antibody | Cat. No. | Supplier | Host | Dilution |
|---|---|---|---|---|
| IF | ||||
| Primary antibodies | ||||
| Endoglin/CD105 | BAF1320 | R&D Systems, Minneapomis, MN | Goat | 1/1000 |
| Kit/CD117 | A4502 | Dako North America, Carpinteria, CA | Rabbit | 1/500 |
| Kit (C-14) | sc-1494 | Santa Cruz Biotechnology, Santa Cruz, CA | Goat | 1/500 |
| CD/31 | 550274 | BD Pharmingen, San Diego, CA | Rat | 1/1000 |
| CD34 | 14-0341 | eBioscience, San Diego, CA | Rat | 1/500 |
| Secondary antibodies | ||||
| Goat Alexa 448 | A11055 | Invitrogen, Eugene, OR | Donkey | 1/200 |
| Rabbit biotinylated | 711065152 | Jackson Immunoresearch, Cambridge, UK | Donkey | 1/200 |
| Rat FITC | 712095150 | Jackson Immunoresearch, Cambridge, UK | Donkey | 1/200 |
| IHC | ||||
| Primary antibodies | ||||
| Endoglin (H-300) | sc-20632 | Santa Cruz Biotechnology, Santa Cruz, CA | Rabbit | 1/100 |
| Secondary antibodies | ||||
| Rabbit biotinylated | 711065152 | Jackson Immunoresearch, Cambridge, UK | Donkey | 1/200 |
| WB | ||||
| Primary antibodies | ||||
| Endoglin/CD105 (mice tissue) | BAF1320 | R&D Systems, Minneapomis, MN | Goat | 1/1000 |
| Endoglin (H-300) (GIST882 cells) | sc-20632 | Santa Cruz Biotechnology, Santa Cruz, CA | Rabbit | 1/500 |
| Gapdh | #2118L | Cell Signaling Technology, Danvers, MA | Rabbit | 1/1000 |
| Secondary antibodies | ||||
| Goat DyLight™ 680 | 35518 | Pierce, Thermo Fisher Scientific, Erembodegem, BE | 1/10000 | |
| Rabbit DyLight™ 800 | 35571 | Pierce, Thermo Fisher Scientific, Erembodegem, BE | 1/10000 | |
| FACS | ||||
| p-Tyr (Alexa Fluor™ 641 conjugated) | #9415 | Cell Signaling Technology Inc., Danvers, MA | 1/50 | |
| Kit/CD117 (R-PE conjugated) | #9415 | BD Pharmingen, San Diego, CA | 1/50 |