Fig 4.

Inhibition of NF-κB pathway by the induction of APR-1 expression in melanoma cells. (A, B) Western blot analysis demonstrates time-dependent inhibition of p-IκBα that is correlated with the increase of IκBα protein stability by the induction of APR-1 in both A375-APR-1and BLM-APR-1 cells. (C, D) EMSA demonstrates time-dependent inhibition of the transcription factor NF-κB by the expression of APR-1 in both A375-APR-1 and BLM-APR-1cells, when compared to control parental cell cultured in the absence or in the presence of Dox. Data are representative of three independent experiments.