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LC3-II interacts with BAG-1. Cytoplasmic extracts of the left ventricular tissue is immunoprecipitated with BAG-1 specific antibodies. BAG-1 immunoprecipitates were separated by SDS-PAGE, and Western immunoblotting was performed with specific antibodies. Results are representative images of three separate experiments. C – Control, I/R – Ischaemia and reperfusion, IA – Ischaemic adaptation followed by I/R, T – cytosolic extract from IA sample.
