Table II.
Primary antibodies for immunohistochemistry and western blotting.
| Antibody (host, clonality, Company) | Purpose | Dilution |
|---|---|---|
| FGFR1 (rabbit, polyclonal, Santa Cruz Biotechnology, CA) | IHC | 1:150 |
| FGFR2 (rabbit, polyclonal, Santa Cruz Biotechnology, CA) | IHC | 1:150 |
| FGF7 (rabbit, polyclonal, Santa Cruz Biotechnology, CA) | IHC | 1:150 |
| FGF7 (rabbit, polyclonal, Abcam, Cambridge, UK) | WB | 1:200 |
| FGF10 (rabbit, polyclonal, Santa Cruz Biotechnology, CA) | IHC | 1:150 |
| FGF10 (goat, polyclonal, Peprotech Inc., Rocky Hill, NJ) | WB | 1:200 |
| p-p44/42 MAPK (Thr202/Tyr204) (rabbit, polyclonal, Cell Signaling, Beverly, MA) | WB | 1:1,000 |
| Akt (rabbit, polyclonal, Cell Signaling, Beverly, MA) | WB | 1:1,000 |
| p-Akt (Ser473) (rabbit, polyclonal, Cell Signaling, Beverly, MA) | WB | 1:1,000 |
| Actin (mouse, monoclonal, Amersham, Buckinghamshire, UK) |
IHC, immunohistochemistry; WB, western blotting.