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. 2008 Oct 13;13(5):985–994. doi: 10.1111/j.1582-4934.2008.00530.x

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© 2009 The Authors Journal compilation © 2009 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd

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Effect of LPS incubation on stimulation of cyclic AMP levels by adenosine (A) and CGS 21680 (B) in human PMN. Human PMN were incubated with either culture medium alone (○) or with medium containing 10 μg/ml LPS (•) for 6 hrs. Hereafter, adenosine (A) and CGS 21680 (B) concentration-effect curves to stimulate accumulation of cAMP were obtained by ELISA. Basal cAMP values were 18 ± 4 and 21 ± 3 pmoles/10⁶ in native and LPS-stimulated PMN, respectively. Corresponding maximal cAMP levels were determined in the presence of 10⁻⁴ M adenosine. Maximal cAMP levels were 85 ± 12 and 76 ± 10 pmoles/10⁶ in native and LPS-stimulated PMN, respectively. Curves are representative of a single experiment measured in triplicates from a series of four independent experiments. Curves were calculated using SigmaPlot 9.0 software. Dashed lines indicate EC50 values.

Effect of LPS incubation on stimulation of cyclic AMP levels by adenosine (A) and CGS 21680 (B) in human PMN. Human PMN were incubated with either culture medium alone (○) or with medium containing 10 μg/ml LPS (•) for 6 hrs. Hereafter, adenosine (A) and CGS 21680 (B) concentration-effect curves to stimulate accumulation of cAMP were obtained by ELISA. Basal cAMP values were 18 ± 4 and 21 ± 3 pmoles/10⁶ in native and LPS-stimulated PMN, respectively. Corresponding maximal cAMP levels were determined in the presence of 10⁻⁴ M adenosine. Maximal cAMP levels were 85 ± 12 and 76 ± 10 pmoles/10⁶ in native and LPS-stimulated PMN, respectively. Curves are representative of a single experiment measured in triplicates from a series of four independent experiments. Curves were calculated using SigmaPlot 9.0 software. Dashed lines indicate EC50 values.