1.
Influence of inflammatory mediators on vitality and on spontaneous and fMLP-stimulated H2O2 production of human PMN: spontaneous and fMLP-stimulated oxidative activity, dead cells after stimulation and number of independent experiments
| Spontaneous activity [MFI] | fMLP [MFI] | PI-positive PMN [%] | n | |
|---|---|---|---|---|
| Control | 31 (± 0.85) | 59 (± 0.85) | 1.52 (± 0.14) | 31 |
| LPS | 33 (± 2.32) | 65 (± 4.88) | 3.91 (± 0.50) | 14 |
| TNF-α | 34 (± 1.51) | 59 (± 4.39) | 2.89 (± 0.39) | 9 |
| IFN-γ | 36 (± 2.37) | 65 (± 4.70) | 2.76 (± 0.37) | 7 |
| IL-1 | 33 (± 1.61) | 62 (± 3.37) | 1.77 (± 0.18) | 12 |
| IL-8 | 30 (± 1.76) | 55 (± 5.57) | 1.74 (± 0.36) | 8 |
Human PMN were incubated in culture medium with different cytokines or without stimulation (control) for 6 hrs. Spontaneous and fMLP (10−7 mol/l)-stimulated H2O2 production and proportion of propidium iodide (PI)-positive cells were assessed by flow cytometric analysis. Data represent the mean (± S.E.M.) of n independent experiments, MFI: mean fluorescence intensity