3.
[3H] ZM 241385 binding to neutrophil membranes of native human PMN, LPS-stimulated human PMN and PMN of sepsis patients: mean (±S.E.M.) KD-values, mean (±S.E.M.) Bmax-values and number of independent experiments
| KD [nM] | Bmax[fmol/mg protein] | n | |
|---|---|---|---|
| Control | 1.02 (± 0.22) | 64.2 (± 17.5) | 6 |
| LPS | 2.48 (± 0.27) | 87.6 (± 26.9) | 6 |
| Sepsis | 1.86 (± 0.32) | 70.2 (±15.5) | 6 |
Membrane fractions from the pre-incubated PMN and from PMN of sepsis patients were prepared and incubated with increasing concentrations of the specific A2A receptor antagonist [3H]-ZM 241385. Non-specific binding was determined in the presence of 1 μM of ZM 241385.