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RT-PCR Primer Sequences to check for A2AR splice variants: sequences, annealing temperatures, expected amplification product lengths and accession number
| Primer name | Sequence | Tm[°C] | Product size [bp] |
|---|---|---|---|
| A2A-splice 3′-For | 5′-GACCGCTACATTGCCATCC-3′ | 58.8 | 298 |
| A2A-splice-3′-Rev | 5′-GCAAATAGACACCCAGCATGA-3′ | 57.9 | |
| A2A-splice 5′-For | 5′-GTGTGGCTCAACAGCAACCT-3′ | 59.4 | 301 |
| A2A-splice 5′-Rev | 5′-ACCCAGCAGATGGCAATGAT-3 | 57.3 |
Accession no. A2AR: NM_000675.
