Fig 3.

ROCK inhibition abrogated PKC-δ and Smad3 activation evoked by TGF-β1. (A) The effect of TGF-β1 (10 ng/ml) on the phosphorylation levels of PKC-δ and Smad3 in the presence or absence of Y-27632 or SB-431542, respectively for 24 hr, was determined by Western blotting, all the blots were normalized by total PKC-δ or Smad3 levels. (B) The effect of TGF-β1 (10 ng/ml) on the transcriptional levels of BMP-2 in the presence or absence of Y-27632 or SB-431542, respectively for 24 hr, was evaluated by quantitative PCR. (C) Western blotting analyses of TGF-β1 (10 ng/ml)-induced activation of PKC-δ and Smad3 with or without treatment of Y-27632 or SB-431542 for 24 hr in BMP-2 knock-down cardiomyocytes. Data were representative as means ± S.E.M. of n = 4 independent experiments. *P < 0.05, versus Control group; #P < 0.05, versus TGF-β1 (10 ng/ml) group.