Fig 6.

MiR-210 is essential for the pro-angiogenic effects of expanded CD34+ cells on HUVEC in vitro tube formation assay. Post-expansion cells were transfected with miR-210 inhibitor or mimic, washed and incubated with HUVEC in matrigel coated 96-well plate. MiR-210 inhibitor resulted in significant decrease in number of branch points by cells expanded with or without VEGF (A: P < 0.0001 and B: P < 0.0001), whereas 210-mimic enhanced tube formation by postEX/noVEGF cells. Upper panel shows representative images of different groups (×40; scale bar = 100 μm). Lower panel represents percentage increase in number of branch points compared to HUVEC alone. **, *** represents post-hoc P values of <0.01, and <0.001 respectively.