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. 2012 Sep 26;16(10):2504–2517. doi: 10.1111/j.1582-4934.2011.01480.x

Fig 3.

Fig 3

Regulation of miR-20b and the effect of antagomiR-20b on its target VEGF and HIF-1a expression: (A) Taqman real-time PCR quantification of miR-20b in ischaemia experimental groups are IR vehicle (VEH) (1), IR + γ-tocotrienol (TOC) (2), IR + resveratrol (RES) (3), IR + γ-tocotrienol + resveratrol (RES+TOC) (4), and IR + longevinex (LONG) (5). *P < 0.05 versus IR vehicle where n = 4/group. All data are normalized and converted to fold change of sham vehicle (BS). (B) VEGF Western blot analyses and its quantification of the above samples. (C) VEGF Western blot analyses and its quantification of the same group of samples when pretreated with antagomiR-20b. *P < 0.05 versus IR Vehicle where n = 4/group. (D) VEGF Western blot analyses and its quatification of the samples IR vehicle (VEH) (1), IR + γ-tocotrienol (TOC) (2), IR + resveratrol (RES) (3), IR + γ-tocotrienol + resveratrol (RES+TOC) (4), and IR + longevinex (LONG) (5). (E) VEGF Western blot analyses and its quatification of the same group when pretreated with antagomiR-20b. *P < 0.05 versus IR vehicle where n = 4/group.