Table 1.
Primer sequences
| Gene | Primer sequence | Size (bp) |
|---|---|---|
| IGF-1 | AGGCTATGGCTCCAGCATTC (F) | 166 |
| AGTCTTGGGCATGTCAGTGTG (R) | ||
| VEGF | ACCCCGACGAGATAGAGTACAT (F) | 200 |
| CTTCTAATGCCCCTCCTTGT (R) | ||
| SDF-1 | CGGCTGAAGAACAACAACAG (F) | 122 |
| GGGGGTCTACTGGAAAGTCC (R) | ||
| FGF-2 | TGTCTATCAAGGGAGTGTGTGC (F) | 154 |
| CAACTGGAGTATTTCCGTGACC (R) | ||
| AKT | CCTCAAGAACGATGGCACCT (F) | 151 |
| CAGGCAGCGGATGATAAAGG (R) | ||
| SIRT-1 | TAGGGACCTTTGCCTCATC (F) | 100 |
| GGCATTCACCACCTAGCC (R) | ||
| p53 | AGCATCTTATCCGGGTGGAAG (F) | 157 |
| CCCATGCAGGAGCTATTACACA (R) | ||
| p21 | GTACTTCCTCTGCCCTGCTG (F) | 171 |
| AGAAGACCAATCTGCGCTTG (R) | ||
| Bax | TGGAGATGAACTGGACAGCA (F) | 152 |
| CAAAGTAGAAGAGGGCAACCAC (R) | ||
| Bak | CAGGACACAGAGGAGGTCTTTC | 182 |
| TAGCGCCGGTTAATATCATCTC | ||
| Bcl-2 | GATGACTTCTCTCGTCGCTACC | 182 |
| ACGCTCTCCACACACATGAC | ||
| p16INK4a | GCTCAACTACGGTGCAGATTC (F) | 196 |
| TCGCACGATGTCTTGATGTC (R) | ||
| PCNA | GGTTGGTAGTTGTCGCTGTA (F) | 720 |
| CAGGCTCATTCATCTCTATCG (R) | ||
| GATA-4 | ACCCATAGTCACCAAGGCTG (F) | 197 |
| ACCCATAGTCACCAAGGCTG (R) | ||
| MEF-2 | CACGCCTGTCACCTAACATCC | 178 |
| TGTTAGGTCTCAAACGCCAC | ||
| NKx2.5 | GGTCTCAATGCCTATGGCTAC (F) | 142 |
| GTTCACGAAGTTGCTGTTGG (R) | ||
| GAPDH | CTCTTGCTCTCAGTATCCTTG (F) | 370 |
| GCTCACTGGCATGGCCTTCCG (R) |