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(A) The effects of 25 μM, 50 μM, and 75 μM Minerval (M25, M50 and M75, respectively) and (B) 25 μM, 50 μM, and 100 μM OA (OA25, OA50 and OA100, respectively) on Jurkat cell number. Cell proliferation was also measured using the MTT method in Jurkat cells treated with the same concentrations of Mineval (C) and OA (D). (E) The effects of 50 μM (M50) and 75 μM (M75) Minerval (48 hrs) on the cellular accumulation of cyclin D3 (Cy) and cdk2 (cdk) as determined by immunoblotting. Tu, tubulin.
