Fig 2.

pH dependence of the relative hMBP-hydrolyzing activity (RA) of individual IgGs from the sera of five different MS patients (graphs 1–5). Hydrolysis of hMBP incubated alone was used as control (‘Con.’) The relative protease activity corresponding to a complete transition of 0.19 mg/ml hMBP to its shorter oligopeptides after 1.5 hrs in the presence of 0.1 mg/ml pIgGs was taken for 100%. The average error in the initial rate determination from two experiments did not exceed 7–10%. For other details see Materials and Methods.