Table 2.

The K_m and k_cat values characterizing interaction of pIgG sub-fractions separated by chromatography on hMBP–Sepharose with hMBP and specific 19-mer oligopeptide

Fraction number*	Condition of elution	Protease activity, hMBP		Protease activity, OP-19
		K m, μM	k cat, min−1	K m, mM	k cat, min−1
1	0.1 M NaCl	5.0 6 1.3	2.3 6 0.3	1.0 6 0.2	156.0 6 30.0
5	1.0 M NaCl	1.5 6 0.2	1.0 6 0.18	0.25 6 0.05	33.0 6 5.0
9	3 M MgCl2	0.9 6 0.015	0.98 6 0.15	0.1 6 0.018	14.3 6 2.5

^*Numbers of fractions corresponds to Fig. 5. For each fraction, a mean of three repeats is used. The k_cat values were calculated as k_cat=V_max/[IgG].