6.

The effect of TQ treatment on the growth pattern of C26 murine colon carcinoma cell spheroids. Spheroids of untreated C26 colon carcinoma cells show a dense growth pattern (A, C) and signs of high proliferation such as cytoplasmatic basophilia, hyperchromasia of the nucleus, enlargement and multiplication of the nucleoli as well as numerous atypical mitosis (E). In contrast, after treatment of C26 spheroids by TQ increased apoptotic features can be observed: loss of intercellular contact, homogenous eosinophil cytoplasma, blebs of the cell membrane, condensation of chromatin as well as fragmentation and loss of the nucleus (B, D, F). (a = apoptosis, b = blebs, f = fragmented nuclei, m = mitosis, n = nucleoli; * loss of intercellular contact; microscope: Nikon Eclypse TE300, camera:Nikon Coolpix 990; x200, x1000).