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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Anal Biochem. 2013 Jul 11;442(1):10.1016/j.ab.2013.06.014. doi: 10.1016/j.ab.2013.06.014

Figure 1. Selected reaction monitoring (SRM) total ion current chromatograms of the targeted oxidized metabolites of linoleic, arachidonic and docosahexaenoic acids.

Figure 1

The indicated fatty acid metabolite standards were derivatized with N-(4-aminomethylphenyl)-pyridinium (AMPP) and measured by LC/MS/MS via SRM in the positive ion mode following chromatographic separation using a C18 reverse phase HPLC column as described in “Materials and Methods”. Representative SRM chromatograms for linoleic acid (LA) and the indicated oxidized LA metabolites (A); arachidonic acid (AA) and the indicated oxidized AA metabolites (B and C); and docosahexaenoic acid (DHA) and the indicated oxidized DHA metabolites (D) are shown with their retention times and the informative SRM transitions.