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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Anal Biochem. 2013 Jul 11;442(1):10.1016/j.ab.2013.06.014. doi: 10.1016/j.ab.2013.06.014

Figure 5. Determination of the false positive identification of LTB4 in a biologic sample utilizing SRM with accurate product ion mass analyses.

Figure 5

(A) Peak retention time profile of LTB4-AMPP standard utilizing SRM (m/z 503 → m/z 391); (B) high mass accuracy extracted ion chromatogram of the product ion, m/z 391.2016 (within an error of 2 ppm) of the standard LTB4-AMPP; (C) corresponding full MS/MS spectrum of the LTB4-AMPP standard; (D) Peak retention time profile indicative of LTB4-AMPP obtained from murine mesenteric arterial tissue utilizing the SRM transition (m/z 503 → m/z 391); (E) high mass accuracy extracted ion chromatogram of the product ion, m/z 391.3355, present in the biologic sample which does not correspond to the accurate mass of the product ion of LTB4-AMPP thereby revealing a false positive identification if only mass unit resolution is utilized (F). The MS/MS spectra of D and E confirm substantial differences between the LTB4 standard and compounds in the biological sample.