Skip to main content
. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Anal Biochem. 2013 Jul 11;442(1):10.1016/j.ab.2013.06.014. doi: 10.1016/j.ab.2013.06.014

Figure 6. Oxidized metabolites of linoleic acid, arachidonic acid and docosahexaenoic acid in hepatic tissues from wild-type (WT) and iPLA2γ Knockout (KO) mice.

Figure 6

Oxidized fatty acid metabolites from murine hepatic tissue were extracted into solvent, isolated by solid phase extraction, derivatized with AMPP and quantitated by LC/MS/MS via selected reaction monitoring (SRM) in the positive ion mode following separation of molecular species using a reverse phase column as described in “Materials and Methods”. Significant decreases in the production of multiple identified oxidized metabolites of LA, DHA and AA as a result of genetic ablation of iPLA2γ are displayed in panels A, B and C, respectively. Values presented are the mean ±S.E. and comparisons were made using Student’s t-test (n=4). *: P< 0.05 level, **: P< 0.01 level.