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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Anal Biochem. 2013 Jul 11;442(1):10.1016/j.ab.2013.06.014. doi: 10.1016/j.ab.2013.06.014

Table 1. Relative response factor (R/R0)a, linearity of measurement, correlation coefficient (r2), limits of quantitation (LOQs)b, extraction yield, and method accuracyc analyses.

Analytes Extraction
Yield (%)		 Accuracy
(% ± SD)		 R/R0 LoQ (pg) Calibrated
range (pg)		 Correlation
coefficient (r2 )		 Internal standard
9-HODE 98 95.0 ± 8.5 1.10 ± 0.11 0.05 0.05–300 0.9977 13-HODE-d4
13-HODE 90 105.0 ± 12.5 1.08 ± 0.08 0.07 0.07–300 0.9956 13-HODE-d4
9-OxoODE 84 86.2 ± 5.0 0.72 ± 0.05 0.5 0.5-1000 0.9990 13-HODE-d4
13-OxoODE 88 97.8.0 ± 7.3 0.88 ± 0.12 1.0 1.0–1000 0.9987 13-HODE-d4
9,10-DiHOME 80 103.0 ± 15.0 1.05 ± 0.15 0.08 0.08-300 0.9991 9,10-DiHOME-d4
12,13-DiHOME 77 94.2.0 ± 3.4 0.99 ± 0.10 0.04 0.04-300 0.9989 12,13-DiHOME-d4
9(10)-EpOME 76 91.5 ± 9.5 0.78 ± 0.10 0.5 0.5-1000 0.9999 9,10-DiHOME-d4
12(13)-EpOME 80 95.0 ± 8.5 0.75 ± 0.11 0.5 0.5-1000 0.9945 9,10-DiHOME-d4
Resolvin D1 75 94.1 ± 10.4 0.40 ± 0.10 5.0 5.0-1000 0.9998 PGE2-d4
Resolvin D2 79 110.0 ± 14.8 0.55 ± 0.12 6.0 6.0-1000 0.9937 PGE2-d4
10,17-
DiHDoHEA		 85 98.0 ± 10.3 0.40 ± 0.08 0.5 0.5-500 0.9960 PGE2-d4
19,20-DiHDPA 103 97.2 ± 7.8 5.80 ± 0.75 0.5 0.5-500 0.9948 PGE2-d4
17-HDoHE 82 95.2 ± 10.1 1.20 ± 0.20 2.0 2.0–1000 0.9950 12-HETE-d8
16-HDoHE 78 99.0 ± 3.5 1.25 ± 0.16 2.0 2.0–1000 0.9984 12-HETE-d8
14-HDoHE 78 93.8 ± 8.8 1.18 ± 0.10 1.0 1.0–1000 0.9989 12-HETE-d8
13-HDoHE 83 105.0 ± 11.2 1.32 ± 0.20 2.0 2.0–1000 0.9995 12-HETE-d8
11-HDoHE 77 101.0 ± 8.5 1.15 ± 0.22 2.0 2.0–1000 0.9966 12-HETE-d8
10-HDoHE 81 88.0 ± 7.8 1.40 ± 0.08 2.0 2.0–1000 0.9984 12-HETE-d8
8-HDoHE 82 90.2 ± 9.9 1.05 ± 0.15 1.0 1.0–1000 0.9919 12-HETE-d8
7-HDoHE 79 102.0 ± 8.5 1.20 ± 0.11 1.0 1.0–1000 0.9963 12-HETE-d8
4-HDoHE 83 108.0 ± 4.5 1.00 ± 0.08 1.0 1.0–1000 0.9994 12-HETE-d8
6keto-PGF1α 85 106.3 ± 8.5 0.95 ± 0.05 0.1 0.1–5000 0.9984 TXB2-d4
TXB2 88 100.2 ± 3.5 1.03 ± 0.03 0.1 0.1–500 0.9990 TXB2-d4
PGF2α 78 98.1 ± 7.1 0.91 ± 0.03 0.4 0.4–500 0.9988 PGE2-d4
PGF1α 80 108.2 ± 5.2 2.10 ± 0.03 0.1 0.1–500 0.9917 PGE2-d4
PGE2 77 99.1 ± 4.2 1.02 ± 0.03 0.3 0.3–500 0.9981 PGE2-d4
PGE1 73 103.1 ± 7.1 1.42 ± 0.11 0.2 0.2–500 0.9972 PGE2-d4
PGD2 73 92.1 ± 10.2 0.66 ± 0.07 0.5 0.5–1000 0.9981 PGE2-d4
LTB4 65 98.8 ± 9.1 1.02 ± 0.02 0.7 0.7–1000 0.9992 LTB4-d4
20-HETE 76 105.1 ± 3.1 2.20 ± 0.10 0.3 0.3–500 0.9977 12-HETE-d8
15-HETE 68 97.2 ± 9.2 1.10 ± 0.18 0.6 0.6–1000 0.9953 12-HETE-d8
12-HETE 66 99.9 ± 8.1 1.00 ± 0.05 0.7 0.7–1000 0.9969 12-HETE-d8
11-HETE 73 97.9 ± 4.2 1.32 ± 0.13 0.5 0.5–1000 0.9973 12-HETE-d8
8-HETE 80 103.1 ± 5.5 1.50 ± 0.12 0.5 0.5–1000 0.9972 12-HETE-d8
5-HETE 70 108.4 ± 7.2 0.82 ± 0.08 0.8 0.8–1000 0.9999 12-HETE-d8
14,15-EET 75 94.3.1 ± 4.4 0.91 ± 0.11 0.8 0.8–1000 0.9989 12-HETE-d8
11,12-EET 80 98.9 ± 6.7 0.80 ± 0.08 0.8 0.8–1000 0.9982 12-HETE-d8
8,9-EET 79 101.0 ± 9.9 0.75 ± 0.14 0.8 0.8–1000 0.9991 12-HETE-d8
5,6-EET 85 106.2 ± 7.7 0.95 ± 0.08 0.5 0.5–1000 0.9990 12-HETE-d8
a

Relative response factor (R/R0) was calculated as the ratio of an equivalent amount of analyte (R) to its internal standard (R0) and expressed as the mean ± standard deviation (N=3).

b

LOQ was defined as 3 × the limit of detection which is expressed as pg injected on column resulting an signal to noise ratio of 3:1.

c

Method accuracies were calculated as the percent deviation from the expected concentrations and expressed as the mean ± standard deviation (n= 3 experiments).