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(A) The presence of TSG101 variants in the cytoplasm or nucleus is summarized. ND, not determined. (B) Nuclear (N) and cytoplasmic (C) fractions of U2OS cells expressing indicated constructs were examined by Western blot analysis. In each pair of samples, empty vector (Vector) was included as a control. Arrowheads indicate positions of TSG101 protein. B23 and β-tubulin serve as markers of nuclear and cytoplasmic fractions, respectively.
