Figure 6. Guanylin production is nearly absent during colitis associated with IL-10 deficiency.

(A) GC-C mRNA is not affected by intestinal inflammation, as measured by realtime RT-PCR. n = 5–6 per group (B) Guanylin gene expression is highly reduced in GC-C^−/−IL-10^−/− colon. n = 5 per group; *p<0.005 (C) Western blotting indicates that guanylin protein expression is suppressed by colonic inflammation in both IL-10^−/− and GC-C^−/−IL-10^−/− mice. On this representative blot, each lane represents a sample from an individual mouse. β-tubulin is shown to demonstrate equal loading within each lane.