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. 2013 Nov 11;8(11):e79431. doi: 10.1371/journal.pone.0079431

Figure 1. The secretions of TNF-α and Mtb-specific TNF-α by ESAT-6 or CFP-10-stimulated PBMCs.

Figure 1

PBMCs obtained from active TB patients (n = 25), LTBI individuals (n = 22), healthy control subjects (n = 10) and non-TB patients (n = 10) were stimulated with ESAT-6 or CFP-10. PBMCs stimulated with medium alone were used as a background control. (A) After 16–20 h of incubation, the supernatant was collected and tested for concentrations of secreted TNF-α by ELISA. (B) Mtb-specific TNF-α was calculated by subtracting background TNF-α secreted by medium-stimulated PBMCs from TNF-α secreted by ESAT-6 or CFP-10-stimulated PBMCs. Median values for each group of participants are represented by a horizontal bar. *p < 0.05, **p < 0.001.