Table 3. Case of whole tissue section with histologically diagnosed cervical intraepithelial neoplasia grade 3 (CIN3; worst lesion), in which multiple HPV genotypes were present.

Block	Analyses on whole tissue sections			Analyses on LCM regions
	Macro-dissected region	Histology	HPV type	Laser-capture micro-dissected regiona	Histology	HPV type
1	1A	CIN2	16, 18	1AIb	CIN1	18
				1AIIb	CIN1	18
				1AIIIb	CIN1	18
	1B	CIN2	16, 39	1BI	CIN2	16, 39
				1BII	CIN2	16
	1C	CIN1	16, 39	1CI	borderline CIN1	16
				1CII	borderline CIN1	negative
				1CIII	borderline CIN1	negative
				1CIV	normal	negative
				1CVc	CIN2	16, 39
				1CVId	CIN1	16
	1D	normal	negative	No LCM	No LCM	No LCM
2	2A	CIN3	16	No LCM	No LCM	No LCM
	2B	CIN3	39	No LCM	No LCM	No LCM
3	3A	CIN3	16, 18	No LCM	No LCM	No LCM
	3B	CIN3	16	No LCM	No LCM	No LCM
	3C	CIN3/CIN2e	16, 18	No LCM	No LCM	No LCM
4	4A	CIN2	16, 39	No LCM	No LCM	No LCM
	4B	CIN3	16	No LCM	No LCM	No LCM

Localized detection of HPV was performed on laser-capture micro-dissected (LCM) regions from the hematoxylin and eosin (H&E) before section.

^aHistological images of micro-dissected regions 1AI, 1AII, 1AIII, 1BI, 1BII, 1CV and 1CVI are shown in Figure 3.

^bThese regions were micro-dissected from the hematoxylin and eosin (H&E) after section instead of the H&E before section.

^cRegion 1CV was micro-dissected from the p16 section. This region corresponds with region 1CI, that was micro-dissected from the H&E before section.

^dRegion 1CVI was micro-dissected from the p16 section. This region corresponds with region 1CIII, that was micro-dissected from the H&E before section.

^eHistological diagnosis was CIN3 in the H&E before section and CIN2 in the H&E after section