Figure 7. Increased prevalence of dysmorphic osteocytes is associated with increased porosity of aged BakΔBaxΔOCN mice.
(A) Representative histologic nondecalcified sections showing viable osteocytes with typical morphology (upper panel) and dysmorphic osteocytes (red arrow heads, lower panel) in the periosteal zone of the femoral cortex from 22-month-old female mice. Scale bar, 10µm. (B) The percentage of dysmorphic osteocytes in endosteal (EZ) and periosteal (PZ) zones of the cortex of 22-mo-old female mice, n=7–8/group. *p<0.05 vs. EZ; #p<0.05 vs. PZ of combined controls. (C) Lacunar and canalicular morphology of cortical osteocytes of 22-month-old mice in the endosteal (“E”) and periosteal (“P”) zones, visualized by acid-etch SEM. Scale bar, 50 µm. Inset: high magnification image of typical lacuna (arrowhead) and canaliculi in the periosteal zone. Scale bar 5 µm; *, cortical void.