Fig 4. The impact of IRAK1 deficiency depends on the TLR stimulus.
Peritoneal macrophages from WT and IRAK1 deficient mice were incubated in the presence of different TLR agonists; TLR4 (ultrapure LPS 100 ng/ml), TLR2 (Pam3CSK4 200ng/ml) or TLR7/8 (Thiazoloquinoline, 2.5 μg/ml). Following 20h incubation, cytokines were measured in media. *Statistically significant difference as compared to WT (p<0.05, Mean ± S.E.M.; n=5–7 animals in each group).