Skip to main content
. 2013 Nov 11;8(11):e79140. doi: 10.1371/journal.pone.0079140

Table 1. Information on primary antibodies used in this study (See also Materials and Methods).

Antibody Immunogen Source Host Dilution
5hmC Raised against 5-hydroxymethylcytidine conjugated to KLH Active motif#39769 R 1∶2,000–1∶10,000
5mC Sheep were immunized with 5-methylcytosine coupled tokeyhole limpet hemocyanin (KLH) Life Span;#LSC64477/18463 S 1∶1,000–5,000
5mC (33D3) Mouse monoclonal antibody (33 D3) was made against5-methylcytosine conjugated to ovalbumin Genway Biotech;#20-003-40005 M 1∶2,000
5caC 5-Carboxylcytosine antibody was raised against 5-carboxylcytidineconjugated to KLH and recognizes 5-carboxylcytosine Active motif# 61230 R 1∶1,00–1,1,000
5fC 5-Formylcytosine antibody was raised against 5-formylcytidineconjugated to KLH and recognizes 5-formylcytosine Active motif#61228 R 1∶100–1∶1,000
Caspase-3 (cleaved) Amino terminal residues adjacent to (Asp175) in human caspase Cell SignalingTechnology; #9661 R 1∶500
Caspase-3 (cleaved) KLH coupled synthetic peptide CRGTELDCGIETD correspondingto amino acids 163–175 of human Caspase 3 R&D system; #AF835 R 1∶500
PAR Poly(ADP-ribose) polymer Trevigen#4336-APC-050 R 1∶500
PAR, mAb (10H) Purified poly(ADP-ribose) EnzoALX-804-220-R100 M 1∶500

M = mouse monoclonal; R = rabbit polyclonal; S = sheep polyclonal.