Figure 4. Oskar protein levels are reduced in Dhc depleted egg chambers.

Egg chambers from flies expressing shRNAs targeting luciferase (A) and dhc (B, C) were stained with antibodies against Dhc (Red) and Oskar protein (Green and grey scale). Oskar protein is abundant at the posterior pole in control egg chambers. However, Dynein depletion results in greatly reduced (59%), or absent (7%) posterior Oskar protein (n=84). A’ and B’ are magnified views of the oocyte posterior in which Oskar is shown in grey scale.