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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Biotechnol Lett. 2013 Jul 27;35(12):10.1007/s10529-013-1301-8. doi: 10.1007/s10529-013-1301-8

Fig. 1. Optimization of the experimental model of chondrogenesis in BMSC on nanofibrous PLLA scaffolds by collagen coating.

Fig. 1

A – Visualization of cell morphology by immunostaining for actin cytoskeleton in hBMSCs cultured on non-coated PLLA scaffolds (Ctrl) or scaffolds coated with collagen type I (Coll I) or type II (Coll II). B,C – Quantitation of cell attachment and spreading of hBMSCs on PLLA scaffolds either non-coated (Ctrl) or coated with collagen type I (C-I) or collagen type II (C-II). B – Cell attachment is shown as fold increased in the number of attached cells compared to control PLLA scaffolds. Collagen coating enhances attachment (3 independent experiments, **, p< 0.01). C – Deposition of cartilaginous glycosaminoglycan (GAG) rich matrix by hBMSCs is enhanced on the collagen type II-coated PLLA scaffolds compared to collagen type I-coating as compared to GAG deposition on control untreated PLLA scaffolds.