Fig. 3.

Knockdown of TFAM in A549 cells attenuated the increases of mtDNA copy number and COX activity caused by 25 cGy α-particles. (A) TFAM expression in wild type, sh-scram, sh-TFAM A549 cells was determined by western blotting. (B) TFAM expression in sh-scram, sh-TFAM A549 cells at 12 h after irradiation. (C) Relative mtDNA copy number in sh-scram, sh-TFAM A549 cells at 0, 24 and 30 h after irradiation. (D) COX and SDH activity in sh-scram, sh-TFAM A549 cells at 24 h after irradiation. (*P < 0.05, **P < 0.01.)