Figure 3. Hypoxia does not increase MiR-138 in skeletal muscle cells.

Murine C2C12 skeletal myoblasts (left panels) or differentiated myotubes (right panels) were subjected to hypoxia for 24 h. Protein extracts were immunoblotted for S100A1 protein expression (upper panels) and expression of MiR-138 by qPCR (lower panels). Expression of Myosin Heavy Chain (MHC) was used to verify differentiation. Experiment was performed 3 times, each in duplicate. *, P<0.05 vs normoxic.