Figure 4. Primary human vascular smooth muscle cells do not change S100A1 nor MiR-138 levels during hypoxia.

A) Primary human microvascular smooth muscle cells (HVSMCs, obtained from the ATCC) were subjected to hypoxia for 24 h. Protein extracts were immunoblotted for Hif1-α (to verify induction of hypoxia), β-actin (to verify equal loading), and S100A1. B) Expression of MiR-138 was by qPCR. Experiment was performed 3 times, each in duplicate.