Figure 5. Specific inhibition of MiR-138 prevents the hypoxia-induced loss of S100A1 in ECs.

A) Primary HMVECs were transfected with the antagomir-138 (or control antagomir) and subjected to chemical hypoxia (CoCl₂, 250 µmol/L) for 24 h before extract preparation and immunoblot analysis. S100A1 expression was normalized to that of β-actin. The experiment was done 3 times, each in duplicate. *, P<0.05 vs control antagomir. B) EA.hy926 ECs were transfected with an antimir-138 (or control) and subjected to either gas hypoxia (1% O₂) or chemical hypoxia (CoCl₂, 250 µmol/L) for 24 h. Luciferase activity in cell lysates was reported as relative light units (RLU) normalized to untreated cells. The experiment was done 3 times, each in triplicate. *, P<0.02 vs antimir-138 treated.