Figure 9. MiR-138 compromises VEGF-stimulated NO production by inhibiting S100A1.

Primary HMVEC cells treated in parallel to those in Figure 8 were starved for 24 h in medium supplemented with 0.2% FBS before being treated with 50 ng/ml VEGF. Supernatants were collected 24 h later and analyzed for nitrate/nitrite levels. The experiment was done 3 times, in duplicate. *, P<0.01 vs no Mimic, #, P<0.01 vs control Adenovirus. Re-expression of S100A1 reverses the MiR-138 induced EC dysfunction.