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. 2013 Nov 11;8(11):e78695. doi: 10.1371/journal.pone.0078695

Figure 2. p38MAPK and Ca++/CaMK pathways mediate CREB phosphorylation induced by Ang II.

Figure 2

Representative immunoblots showing the effect of (A) pretreatment with SB203580 (10 µM) followed by Ang II (100 nM) activation on p-CREB levels over a specified time course. (B) pretreatment with KN62 (10 µM) followed by Ang II (100 nM) at 1 hour on the phosphorylation of CREB. (C) CATH.a cells were preincubated for 30 minutes with PD98059 (25 µM), SB203580 (20 µM) and KN62 (10 µM) followed by Ang II (100 nM) for 8 hours. Cell lysates were immunoblotted for p-CREB. (D) Cells were preincubated with or without Losartan (1 µM) followed by Ang II (100 nM) for 8 hours. At the end of the specified time period, cell lysates were tested for p-CREB levels by western blot. *p<0.05 vs control, #p<0.05 vs 1 hr Ang II and †p<0.05 vs corresponding Ang II group ‡p<0.05 vs 8 and 24 hr Ang II treatment. Values are expressed as mean ± SEM. n = 3–4.