Figure 4. Ang II increases CREB and NF-κB activity in an AT1R and CREB-dependent manner.

CATH.a neurons were transfected with CREB and NF-κB reporter constructs, consisting of a mixture of CREB and NF-κB - responsive luciferase constructs and a constitutively expressing Renilla construct. (A) NF-κB relative luciferase activity was quantified under several experimental conditions with Ang II treatment alone or pretreatment with losartan, anti-CREB siRNA or mock transfected cells. (B) CREB luciferase activity was measured under the similar experimental conditions. Values are expressed as mean ± SEM. *p<0.05. n = 3.