Table 3. HO activity assay.
Plant | Zheng58 | Mo17 | elm2 | F2/green | F2/yellow |
---|---|---|---|---|---|
Enzyme Activity (U mg protein-1) | 9.31±0.70 | 8.57±0.32 | 6.35±0.59* | 10.70±0.52 | 6.82±0.68* |
One unit of activity was calculated as the quantity of enzyme needed to produce 1 nM BV per 30 min 37°C. Mean and SE values were calculated from four independent determinations. Asterisk indicates significant difference as compared with Zheng58 wild-type or F2 individuals with green phenotype at P<0.01 (Student’s t test).