| Primer name |
Target a, b
|
Sequence (5'- 3') |
| Primers for generation of directed mutants |
| PBpR412_L |
SpecR cassette |
GCCGCTCTAGAACTAGTGG
|
| PBpR412_R |
SpecR cassette |
GATACCCCTCGAATTGACGC
|
| PBTnMr9 |
SpecR cassette control primer |
CAATGGTTCAGATACGACGAC
|
| SPD_2068_L1 |
htrA left flanking region |
TTCCCTTCAATGGCTAACAC
|
| SPD_2068_L2 |
htrA left flanking region |
CCACTAGTTCTAGAGCGGCAAACTACCCAAGGCTCCAC
|
| SPD_2068_R1 |
htrA right flanking region |
GACTTGCCCATCTTATCTGC
|
| SPD_2068_R2 |
htrA right flanking region |
GCGTCAATTCGAGGGGTATCACCATTCTATCGGAGACACC
|
| SPD_2068_C |
htrA gene control primer |
TCGCTGAGAATCTGTGTCAG
|
| SPD_1753_L1 |
sfp left flanking region |
CCTCTTGGATTAGAGACAATC
|
| SPD_1753_L2 |
sfp left flanking region |
CCACTAGTTCTAGAGCGGCTTTCGCTAGTCTGAGTGTG
|
| SPD_1753_R1 |
sfp right flanking region |
TAGTTGTGGTAACCTGTTTGC
|
| SPD_1753_R2 |
sfp right flanking region |
GCGTCAATTCGAGGGGTATCATTACAAGTCAGTTTAGTTG
|
| SPD_1753_C |
sfp gene control primer |
CTTTCGCCTCCACCAGTAAC
|
| SPD_0558_L1 |
prtA left flanking region |
TTCAAACCACGTCAACGTCG
|
| SPD_0558_L2 |
prtA left flanking region |
CCACTAGTTCTAGAGCGGCTGCAGCTGTAGTTAGTGAC
|
| SPD_0558_R1-2 |
prtA right flanking region |
TAACCGTCCAATAGACTTCG
|
| SPD_0558_R2 |
prtA right flanking region |
GCGTCAATTCGAGGGGTATCAGCCCAGACACTATTAGCTG
|
| SPD_0558_C |
prtA gene control primer |
GTGTCTGCTAAGACTACCTC
|
