Fig. 1.

Effects of nectandrin A on BMP-2-inudced osteoblastic differentiation and mineralization in C2C12 cells. (A) Cells were cultured in a 96-well plate for 24 h and then the medium was replaced with DMEM containing 5% FBS and rhBMP-2 (100 ng/ml) in the presence or absence of nectandrin A (differentiation day 0). Cell viability assay was then performed on the differentiation day 3. Medium was changed every third day. (B) ALP enzyme activity and Alizarin Red staining were performed on the differentiation day 7 and 14, respectively. (C, D) ALP activity and calcium contents were measured at different time points after the induction of C2C12 differentiation in the presence or absence of nectandrin A. ^*p<0.05 and ^**p<0.01 compared to rhBMP-2 treated group.