Table 2. HSA-Flag-TWEAK modulates the production of cytokines in the infarcted heart after MI.
| PBS | HSA-Flag-TWEAK | ||||
| abbreviation/gene name | expression [pg/ml] | SEM | expression [pg/ml] | SEM | p |
| BLC/B-lymphocyte chemoattractant | 116,18 | 5,54 | 110,40 | 4,81 | N.S. |
| CD40 | 10,18 | 0,64 | 12,82 | 1,35 | N.S. |
| CXCL16/Chemokine (C-X-C motif) ligand 16 | 0,50 | 0,30 | 0,43 | 0,43 | N.S. |
| Eotaxin | 10,52 | 0,31 | 11,63 | 0,36 | N.S. |
| Eotaxin-2 | 15,26 | 0,43 | 16,09 | 0,52 | N.S. |
| E-Selectin | 40,71 | 2,92 | 45,90 | 5,04 | N.S. |
| GITR/glucocorticoid induced tumornecrosis factor receptor family related gene | 64,45 | 10,41 | 99,69 | 4,95 | p<0.05 |
| GM-CSF/Granulocyte macrophagecolony-stimulating factor | 2,54 | 1,19 | 2,70 | 1,61 | N.S. |
| ICAM-1 | 420,03 | 107,50 | 365,54 | 186,63 | N.S. |
| IFN-γ | u.d. | 7,47 | 5,75 | p<0.05 | |
| IL-12/Interleukin-12 | u.d. | 44,51 | 3,23 | p<0.05 | |
| IL-12p40/70 | 90,43 | 40,35 | 73,85 | 45,21 | N.S. |
| IL-2/Interleukin-2 | 6,08 | 1,65 | 6,22 | 1,91 | N.S. |
| IL-3/Interleukin-3 | 0,27 | 0,26 | 1,04 | 0,77 | N.S. |
| IL-5/Interleukin-5 | 4,13 | 2,66 | 18,75 | 5,05 | p<0.05 |
| IL-9/Interleukin-9 | 241,45 | 16,66 | 291,12 | 16,77 | N.S. |
| KC/keratinocyte-derived chemokine | 1,29 | 0,72 | 2,89 | 0,58 | N.S. |
| MCP-1/monocyte chemotactic protein-1 | u.d. | 21,54 | 7,27 | p<0.05 | |
| MCP-5/monocyte chemoattractant protein-5 | u.d. | 7,36 | 3,65 | p<0.05 | |
| MDC/macrophage-derived chemokine | 19,18 | 1,99 | 22,49 | 1,54 | N.S. |
| MIP-1γ/macrophage inflammatory protein γ | 412,09 | 117,66 | 747,38 | 107,42 | N.S. |
| MIP-2/macrophage inflammatory protein-2 | 1,41 | 0,88 | 7,83 | 3,22 | N.S. |
| MIP-3α/macrophage inflammatory protein-3α | 7,49 | 0,36 | 7,67 | 0,39 | N.S. |
| OPG/Osteoprotegerin | 39,80 | 2,60 | 49,27 | 3,46 | N.S. |
| Osteopontin | 17780,54 | 5216,12 | 22152,46 | 4037,31 | N.S. |
| PF4/platelet factor 4 | 785,01 | 69,99 | 1362,01 | 337,63 | N.S. |
| P-Selectin | 363,30 | 40,92 | 551,42 | 105,86 | N.S. |
| RANTES/regulated on activation, normalT cell expressed and secreted | u.d. | 4,63 | 3,34 | p<0.05 | |
| Resistin | 34,46 | 5,18 | 43,76 | 16,07 | N.S. |
| sTNFRII | 14,77 | 0,94 | 17,12 | 1,38 | N.S. |
| VCAM-1/vascular cell adhesion molecule 1 | 168,49 | 23,01 | 257,27 | 43,26 | N.S. |
| VEGF-D/vascular endothelial growth factor D | 91,54 | 12,95 | 84,96 | 5,79 | N.S. |
u.d. = under detection limit.
IFN-γ: <11 pg/ml; IL-12: <5 pg/ml; MCP-1: <5 pg/ml; MCP-5: <1 pg/ml; RANTES: <3 pg/ml.
A cytokine array for the detection of the protein expression of different cytokines was performed 3 days after MI. HSA-Flag-TWEAK treated mice showed statistically significant up-regulation of interleukin 12 (IL-12), interleukin 5 (IL-5), monocyte chemotactic protein-1 (MCP-1), macrophage inflammatory protein-2 (MIP-2) and regulated on activation, normal T cell expressed and secreted (RANTES) (u.d. = under limit of detection).