Table 2. Presence of FLT3-ITD and mutated NPM1 in ALDH^bright and ALDH^low compartments in CD34-positive AML.

Patient # FLT3-ITD	CD34– cells	CD34+CD38 ALDHlow	CD34+CD38– ALDHbright
575	pos	50% ITD	wt
808	pos	50% ITD	wt
951	pos	42% ITD	wt
966	pos	43% ITD	wt
1263	pos	60% ITD	wt
Patient # NPM1	CD34– cells	CD34+CD38 ALDHlow	CD34+CD38 ALDHbright
575	mut	mut	wt
808	mut	mut	wt
945	mut	mut	wt (<5%)
670	mut	mut	wt

Detection of molecular aberrancies in CD34+CD38– ALDH^bright and ALDH^low compartments of CD34-positive AML. The FLT3-ITD percentage is determined in the total leukemic blast population (data not shown), the CD34– cell population and the CD34+CD38-ALDH^low and CD34+CD38-ALDH^bright compartments. The NPM1 mutation analysis is not quantative. # the ALDH^bright compartment contained in one case a small mutant peak (AML-945), likely caused by relatively poor separation of ALDH^bright and ALDH^low populations due to overlap in the boundary region.