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. 2013 Nov 11;8(11):e78837. doi: 10.1371/journal.pone.0078837

Figure 4. Structure of the brain cDNA library clone showing specific interaction with ALS4 L389S amino-terminal senataxin.

Figure 4

Here we see a diagram of the fusion clone of BCYRN1 reverse complement (BCYRN1-rc) and KIF1B cDNA sequences, with subclones of BCYRN1-rc and KIF1B coding exons 6–9 shown above the clone map (A), and relative length of relevant clone sequence in base pairs (B). Below is shown the minimal open reading frame peptide product capable of binding L389S senataxin, contained within the BCYRN1 sequence (C). The unique protein fragment highlighted in red text has near perfect homology with several human proteins with brain specific expression. When aligned by NCBI Blast, the 138 bp clone sequence (Query) shows 99% identity with BCYRN1 trancsript NR_001568.1 (Sbjct) over a 110 bp stretch (D).