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. 2013 Nov 11;8(11):e79047. doi: 10.1371/journal.pone.0079047

Figure 2. In presence of high numbers of non-target cells CD19ds-LV is specific for CD19+ cells.

Figure 2

CD19−/CD8+ Molt 4.8 cells were mixed in the indicated ratios with CD19+/CD8− Raji cells and transduced with CD19ds-LV or VSVG-LV vector particles at an MOI of 2 (refers to the total cell number). After 72 h, the cells were stained for CD8 expression and the percentage of EGFP+/CD8− cells was determined by FACS analysis. A gating strategy was chosen in which the untransduced cells lay well within the EGFP-negative quadrants as shown in the upper left diagram.