Figure 5. Synthesized inhibitors of Cdk4 protect neuronally differentiated PC12 cells against NGF deprivation.

Cells were subjected to NGF deprivation in presence and absence of synthesized small molecule inhibitors of Cdk4 for 20(A) Graphical representation of percentage of viable cells following NGF deprivation in presence of synthesized small molecule Cdk4 inhibitors. Doses of each molecule are 8A: 5 µM; 8B: 1 µM; 8C: 1 µM; 8D: 5 µM; 12E: 1 µM; 12F: 5 µM; 12G: 1 µM; 13H: 1 µM; 12I: 1 µM; 13J: 1 µM. Data represented as mean ± SEM of three independent experiments performed in duplicates. The asterisks denote statistically significant differences between indicated class: **p<0.01, *p<0.05. (B) Dose kinetics of synthesized small molecule inhibitor 8A in differentiated PC12 cells in presence and absence of NGF. Data represented as mean ± SEM of three independent experiments. The asterisks denote statistically significant differences between indicated classes: *p<0.05. (C) Dose kinetics of synthesized small molecule inhibitor 8B in differentiated PC12 cells in presence and absence of NGF. Data represented as mean ± SEM of three independent experiments. The asterisks denote statistically significant differences between indicated classes: *p<0.05. (D) Representative phase contrast micrographs show retention of neuronal processes even after NGF deprivation in presence of synthesized Cdk4 inhibitors (8A & 13H).